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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: J Immunol. 2012 Sep 7;189(8):3914–3924. doi: 10.4049/jimmunol.1201604

Figure 6. ATX induces motility of na.

Figure 6

ïve T cells.

Naïve GFP+ CD4+ OTII T cells were seeded onto a crawling chamber coated with 3 μg/ml ICAM-1 + 400 ng/ml CCL21 with or without 1 μg/ml ATX plus 10 μM LPC in solution. T cells were imaged every 15 sec for 15 min and tracked by Imaris software. Cell tracks are shown for a 10 min period on (A, B) an ICAM-1 plus CCL21 substratum and on (C, D) an ICAM-1 plus CCL21 substratum in the presence of 1 μg/ml ATX and 10 μM LPC in solution. A and C show all of the tracks (> 100 cells per experimental set); B and D show 10 randomly chosen sample tracks for a 10 min period of migration referenced to the origin of each cell. Median velocity (E), turning angle (F), and average total displacement (G) of each cell were measured over 10 min and are shown in dot (top) and relative frequency plots (bottom). *** denotes p < 0.001 for the indicated comparison as determined by ANOVA. Data are representative of 3 independent experiments.