Figure 4.

Omeprazole disturbed apical acidity of Caco-2 monolayers. Accumulative apical pH values at various time points after replacing media with fresh one for 14d control cells (A). Apical pH of the media of control and omeprazole exposed Caco-2 cells at 16 h, 20 h, and 24 h (B) after media change. Passive Mg²⁺ transport (40 mmol/L concentration gradient) of control and omeprazole-treated groups that were incubated with CaSR agonists neomycin or spermine (C). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs control group. For each data point, n = 6.