Figure 6.

Apical acidity enhanced passive Mg²⁺ absorption and Cld-7 and -12 expression. Representative time line for apical acidic media treatment (A). Passive Mg²⁺ transport (40 mmol/L concentration gradient) of control monolayers (B). %maximum passive Mg²⁺ transport - Mg²⁺ concentration curve of control monolayers at pH 7.4, 6.5, and 5.5 (C). EC₅₀ of the dose - response curve of control monolayers at pH 7.4, 6.5, and 5.5 (D). For each data point, n = 6. Immunobloting and densitometric analysis of Cldn-7 (E) and -12 (F). For each data point, n = 5. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs control group (n = 5).