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. Author manuscript; available in PMC: 2013 Nov 27.
Published in final edited form as: ACS Nano. 2012 Oct 1;6(11):10168–10177. doi: 10.1021/nn3039148

Figure 2.

Figure 2

Adhesion and growth of human embryonic stem cells on defined hydrogels presenting integrin or glycosaminoglycan-binding peptides. A. Bright field images of human embryonic stem (hES) cell (H9) after 24 h culture on hydrogels functionalized with CGRGDS (integrin ligand) or CGKKQRFRHRNRKG (GAG ligand). Hydrogels were functionalized with a 1:1 ratio of aminomaleimide to glucamine. B. Immunostaining of hES cells (H9) after 7 days of culture on hydrogels presenting either the CGRGDS or CGKKQRFRHRNRKG peptide. Cells were immunostained for pluripotency markers Oct-4 (green) and SSEA-4 (red), and counterstained with DAPI (blue). Scale bars: A: 100 μm, B: 500 μm.