Figure 3.

Assay of enzyme activities in each segment of leek epidermis compared with hentriacontan-16-one accumulation. Segments indicated along x axis are as described in Figure 1. A, Fatty acid elongase activity. B, FAS activity. C, Acyl-ACP-hydrolyzing activity. Solid bars, 16:0-ACP hydrolysis; open bars, 18:0-ACP hydrolysis; and hatched bars, 18:1-ACP hydrolysis. Hentriacontan-16-one (•) was used as a marker for wax load in each experiment. The segment containing ≥ 2 nmol/cm² hentriacontan-16-one was segment V. Mean values with ses are presented. In A and B, n = 7, and in C, n = 2 to 4.