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. Author manuscript; available in PMC: 2012 Nov 28.
Published in final edited form as: J Cell Biochem. 2011 Sep;112(9):2383–2391. doi: 10.1002/jcb.23161

Fig. 1.

Fig. 1

Disappearance of FANCD2 occurs following treatment of cells with DNA damaging agents. HeLa monolayer cells with 50% confluence were treated with 1 µM mitomycin C (MMC, panel A), 100 µM cisplatin (CDDP, panel B), or 100 J/m2 germicidal UV light (panel C). Cells were harvested at various time points and analyzed for FANCD2 by Western blot. FANCD2-L and FANCD2-S represent monoubiquitinated and non-ubiquitinated forms of FANCD2, respectively. Ku70 was used as an internal loading control for disappearance of FANCD2.