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. Author manuscript; available in PMC: 2012 Nov 28.
Published in final edited form as: J Cell Biochem. 2011 Sep;112(9):2383–2391. doi: 10.1002/jcb.23161

Fig. 5.

Fig. 5

Disappearance of FANCD2 correlates with cell death following MMC treatment. A: Disappearance of FANCD2 in WT fibroblast cells following treatment with various concentrations of MMC for 72 h. Cell extracts were subjected to SDS–PAGE and immunoblot using an anti-FANCD2 antibody (top panel). Relative amount of FANCD2 protein (%) was obtained from the Western blot using the NIH image system (version 1.62). Ku70 (bottom panel) was used as an internal loading control. B: Apoptotic cell death (% TUNEL+ cells) of WT and FANC mutant cells following MMC treatment. Values are the average (±SEM) of three separate experiments. P <0.01; *P=0.05; **P=0.07.C: Disappearance of FANCD2 protein correlates with apoptotic cell death following MMC treatment.