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. Author manuscript; available in PMC: 2012 Nov 29.
Published in final edited form as: Nat Med. 2011 Dec 18;18(1):159–165. doi: 10.1038/nm.2559

Figure 4. Sirt1 rescues mutant HTT-mediated interference with TORC1 activity.

Figure 4

(a) Immunostaining of HTT, TORC1 and DAPI in primary neurons infected with lentiviral WT HTT (upper panels) or mt HTT (lower panels). *** P < 0.001 by t-test. Scale bar, 50 µm. (b) Chromatin immunoprecipitation (ChIP) of TORC1 on BDNF in cortex of WT or HD mice. **P < 0.01 vs WT, n = 6. (c) Coimmunoprecipitations assay of indicated plasmids in N2a cells. (d) BDNF promoter IV activities in N2a cells cotransfected with indicated plasmids at 48 hooo posttransfection. ** P < 0.01. (e) NF staining intensity in primary cortical neurons infected with indicated lentivirus. * P < 0.05 by t-test. *** P < 0.001 by t-test. (f) Toxicity was assessed as in (e). * P < 0.05, *** P < 0.001 by t-test.