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Viral replication was assessed by one-step viral growth curves. Representative results in GSC deriving from GBM 6, 10 and 12 are shown. Cells were infected with MV-NIS, MV-GFP or MV-CEA at an MOI of 1, harvested at different time points and titered in Vero cells. Results are expressed as TCID50 of harvested virus. All glioma stem cell lines effectively supported viral replication.
