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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Neurochem. 2012 Sep 28;124(1):4–14. doi: 10.1111/j.1471-4159.2012.07908.x

Figure 1. Glutaminase is reduced in APOE-ε4 TR mice.

Figure 1

4–5 month old APOE TR mouse brains were homogenized by TBS and then TBS-X by sequential fractionation. Homogenates were probed for Glutamine Synthetase (A–C) and Glutaminase (D–F). (A) A representative immunoblot showing that Glutamine Synthetase is present in both the TBS and TBSX fraction of these mice (upper panel). A representative blot showing the levels of Glutamine Synthetase in the TBSX Fraction (middle panel) and the TBS fraction (lower panel). Tubulin was used as a loading control. (B) Quantification of Glutamine Synthetase levels in the TBS-X and (C) TBS fractions. (D) Representative immunoblot showing that glutaminase is present in the TBSX and TBS fractions. (E) Quantification of the Glutaminase levels in the TBSX and (F) TBS fractions. n=5 per condition. *, p<0.05 compared to APOE-ε3 TR mice. Error bars expressed as S.E.M.