Fig. 2.

Different patch configurations. (A) When the recording pipette is sealed onto the cell, a cell-attached patch is achieved. Rapid excision results in an inside-out patch (left). Suction results in a whole-cell patch (right) where gentle excision results in an outside-out patch. (B) Use of nystatin in a procedure similar to the one shown in A results in formation of a perforated patch or, with gentle excision, a perforated vesicle.