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. 2012 Nov 17;8(10):1431–1446. doi: 10.7150/ijbs.4664

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 3 — SMC and HuVEC cell cycle detection during CO exposure by flow cytometry. After SMC and HuVEC were exposed to 250 ppm of CO for 6 h, cell percentage was analyzed in different cell cycle phase on SMC and HuVEC in the presence and absence of CO. (A) Representative flow cytometric analysis on SMC in G0/G1, S and G2/M phase in normal culture condition. (B) Representative flow cytometric analysis on SMC in G0/G1, S and G2/M phase in exposure to 250 ppm of CO condition for 6 h. (C) There was a significant difference in cell percentage in G0/G1 and S phase between SMC and CO-treated SMC (p=0.043). (D) Representative flow cytometric analysis on HuVEC in G0/G1, S and G2/M phase in normal culture condition. (E) Representative flow cytometric analysis on HuVEC in G0/G1, S and G2/M phase in exposure to 250 ppm of CO condition for 6 h. (F) There was also a significant difference in cell percentage in G0/G1 and S phase between HuVEC and CO-treated HuVEC (p=0.039).