Table 3.
Avoidance conditioning-induced regulation of protein abundances in synaptic cytomatrices
| Swissprot ID | Gene description | Brain region | Time point | Regulation* | ||
|---|---|---|---|---|---|---|
| AV/NV | FS/NV | TS/NV | ||||
| Cytoskeleton and scaffolding proteins | ||||||
| BSN_MOUSE | Protein bassoon | STR | 6 h | 0.52* | 0.57 | 0.72 |
| DYH5_MOUSE | Dynein heavy chain 5, axonemal | FC | 24 h | 0.66* | 1.08 | 1.02 |
| DYH8_MOUSE | Dynein heavy chain 8, axonemal | STR | 24 h | 0.64* | 0.89 | 0.89 |
| MACF1_MOUSE | Microtubule-actin cross-linking factor 1 | HIP | 24 h | 0.55* | 1.18 | 0.74 |
| MAP1A_MOUSE | Microtubule-associated protein 1A | FC | 24 h | 0.48* | 1.06 | 0.84 |
| MY18A_MOUSE | Myosin-XVIIIa | AC | 24 h | 0.57* | 1.01 | 1.45 |
| MYO5A_MOUSE | Myosin-Va | AC | 6 h | 0.65* | 1.55 | 1.12 |
| MYO5A_MOUSE | Myosin-Va | AC | 24 h | 0.49* | 0.95 | 0.47 |
| NFH_MOUSE | Neurofilament heavy polypeptide | AC | 24 h | 0.46* | 1.47 | 1.28 |
| NRAP_MOUSE | Nebulin-related-anchoring protein | FC | 24 h | 0.64* | 1.32 | 1.10 |
| OBSCN_MOUSE | Obscurin | HIP | 6 h | 0.54* | 1.42 | 1.13 |
| PCLO_MOUSE | Protein piccolo | FC | 24 h | 0.49* | 0.77 | 0.77 |
| PCLO_MOUSE | Protein piccolo | STR | 24 h | 0.50* | 0.86 | 0.79 |
| PLEC1_MOUSE | Plectin-1 | FC | 6 h | 0.75* | 1.01 | 0.75 |
| SPTA2_MOUSE | Spectrin alpha chain, brain | FC | 6 h | 0.68* | 1.03 | 1.12 |
| SPTB2_MOUSE | Spectrin beta chain, brain 1 | AC | 24 h | 0.59* | 0.88 | 0.70 |
| SPTB2_MOUSE | Spectrin beta chain, brain 1 | FC | 24 h | 0.59* | 1.45 | 1.56 |
| SPTB2_MOUSE | Spectrin beta chain, brain 1 | STR | 24 h | 0.48* | 0.79 | 0.66 |
| TITIN_MOUSE | Titin | AC | 6 h | 0.62* | 0.90 | 0.98 |
| TITIN_MOUSE | Titin | AC | 24 h | 0.54* | 1.03 | 0.94 |
| TITIN_MOUSE | Titin | FC | 6 h | 0.61* | 1.09* | 0.85 |
| TITIN_MOUSE | Titin | FC | 24 h | 0.57* | 0.98 | 0.88 |
| TITIN_MOUSE | Titin | HIP | 24 h | 0.67* | 1.04 | 0.97 |
| TITIN_MOUSE | Titin | STR | 6 h | 0.67* | 1.06 | 1.10 |
| TITIN_MOUSE | Titin | STR | 24 h | 0.60* | 0.93 | 0.83 |
| TPPP_MOUSE | Tubulin polymerisation-promoting protein | AC | 6 h | 0.44* | 1.36 | 0.83 |
| Extracellular matrix | ||||||
| CO4A4_MOUSE | Collagen alpha-4(IV) chain | FC | 24 h | 0.75* | 1.00 | 0.86 |
| COBA2_MOUSE | Collagen alpha-2(XI) chain | HIP | 24 h | 0.46* | 0.81 | 0.71 |
| COGA1_MOUSE | Collagen alpha-1(XVI) chain | FC | 6 h | 0.63* | 0.81 | 0.60 |
| Trafficking/endocytosis | ||||||
| HRBL_MOUSE | HIV-1 Rev-binding protein-like protein | AC | 24 h | 0.60* | 0.97 | 0.95 |
| HRBL_MOUSE | HIV-1 Rev-binding protein-like protein | FC | 6 h | 0.64* | 0.87 | 0.89 |
| HRBL_MOUSE | HIV-1 Rev-binding protein-like protein | HIP | 24 h | 0.67* | 0.91 | 0.78 |
| HRBL_MOUSE | HIV-1 Rev-binding protein-like protein | STR | 24 h | 0.79* | 0.99 | 0.88 |
| U119B_MOUSE | Protein unc-119 homolog B | STR | 6 h | 0.66* | 0.94 | 0.94 |
| U119B_MOUSE | Protein unc-119 homolog B | STR | 24 h | 0.59* | 0.62 | 0.73 |
| Ubiquitination | ||||||
| CBL_MOUSE | E3 ubiquitin-protein ligase CBL | AC | 6 h | 0.73* | 1.00 | 1.04 |
| CBL_MOUSE | E3 ubiquitin-protein ligase CBL | AC | 24 h | 0.64* | 0.98 | 0.96 |
| CBL_MOUSE | E3 ubiquitin-protein ligase CBL | FC | 24 h | 0.64* | 1.08 | 0.92 |
| CBL_MOUSE | E3 ubiquitin-protein ligase CBL | STR | 6 h | 0.70* | 0.87 | 0.97 |
| DNA and RNA binding/transcription/translation | ||||||
| GAS8_MOUSE | Growth arrest-specific protein 8 | FC | 24 h | 0.68* | 0.95 | 0.86 |
| H2AW_MOUSE | Core histone macro-H2A.2 | STR | 24 h | 0.49* | 0.58 | 1.32 |
| H4_MOUSE | Histone H4 | AC | 6 h | 0.64* | 1.02 | 0.85 |
| H4_MOUSE | Histone H4 | AC | 24 h | 0.67* | 0.87 | 0.82 |
| H4_MOUSE | Histone H4 | FC | 24 h | 0.51* | 0.97 | 0.99 |
| H4_MOUSE | Histone H4 | STR | 24 h | 0.52* | 1.00 | 0.81 |
| HNRPM_MOUSE | Heterogeneous nuclear ribonucleoprotein M | FC | 6 h | 0.71* | 1.28 | 1.10 |
| HNRPM_MOUSE | Heterogeneous nuclear ribonucleoprotein M | STR | 6 h | 0.45* | 0.78 | 0.70 |
| LSD1_MOUSE | Lysine-specific histone demethylase 1 | STR | 24 h | 0.44* | 0.66 | 0.61 |
| NUCL_MOUSE | Nucleolin | STR | 24 h | 0.47* | 0.64 | 0.70 |
| PIN4_MOUSE | Peptidyl-prolyl cis-trans isomerase | STR | 6 h | 0.73* | 0.94 | 1.05 |
| SLTM_MOUSE | SAFB-like transcription modulator | FC | 24 h | 0.67* | 0.82 | 0.85 |
| SRP68_MOUSE | Signal recognition particle 68 kDa protein | AC | 6 h | 0.67* | 0.91 | 0.95 |
| Kinases | ||||||
| ROCK2_MOUSE | Rho-associated protein kinase 2 | FC | 24 h | 0.35* | 0.47 | 0.74 |
a) Mean protein abundances in PSD-enriched fractions derived from trained mice (AV), foot-shock controls (FS), and tone controls (TS) normalised to the corresponding values from naïve mice (NV).
*
p < 0.05, significantly different from 1 (one sample t-test; 3≤n≤4 per group).