Figure 1.

Quantitative analysis of vlx and vsp steady-state mRNA levels in sink-regulated soybean leaves from plants with pods removed daily for 6 weeks. a, Compilation of autoradiographs from a representative RNase-protection analysis. Pod removal began 1 week after anthesis with plants 9 weeks old. Selected trifoliate leaflets were harvested beginning at the time of first pod removal (Week 1) and at weekly intervals thereafter (Weeks 2–6). Total RNA was extracted and mRNA analyzed using 5 μg of total RNA and 2 fmol ³²P-labeled antisense riboprobe specific to 5′ domains of vlx genes A through E and to vspB. Hybridization products were then separated on polyacrylamide gels. Only the portions of the gels representing full- length riboprobes protected by specific mRNAs are shown. b, Quantitation of vlxA through vlxE and vspB steady-state transcript from phosphor-image analysis of the ³²P label in full-length protected bands from independent RNase-protection assays. The scale varies for each riboprobe. Error bars show sd (n = 2). c, Display of data described in Figure 1b, but at a common vertical scale to demonstrate comparative base levels of transcript accumulation and subsequent responses.