Fig. 3. DG1 regulates somatic muscle patterning and myotube extension with Gyc76C.

In stage 16 wild-type embryos (A), VO (A, arrows) and VA (A, arrowheads) muscles extend towards the ventral midline. In pkg21D^f05504 mutant embryos (B) and embryos trans-heterozygous for pkg21D^f05504 and gyc76C²³⁸⁸ (C), VO muscles are not as extended (B,C, arrows). In embryos expressing pkg21D RNAi specifically in the muscle with twi-GAL4; mef2-GAL4 (D), VO (D, large arrow) and VA (D, arrowheads) muscles are thinned and unfused myoblasts are present (D, small arrows). Embryos shown were stained for β3t which labels all somatic muscles as well as hemocytes (asterisks in D) in the vicinity. Scale bar in A represents 20 µm.