Fig. 5. gyc76C and pkg21D control integrin localization at the MTJ.

In gyc76C²³⁸⁸ heterozygous embryos (A), βPS integrin (A′,A″, white) and F-actin (A, green) accumulate at MTJs (A″, arrow) and in intracellular puncta (A″, arrowhead). In embryos homozygous for gyc76C²³⁸⁸ (B) or gyc76C^Ex173 (C), and embryos expressing RNAi to gyc76C (D) or pkg21D (E) specifically in the muscle with twi-GAL4; mef2-GAL4, βPS (B′–E′,B″–E″) is diffused at the MTJs (B″–E″, arrows) and is found as intracellular puncta in the dorsal muscles (B″–E″, arrowheads). In gyc76C²³⁸⁸ homozygous embryos expressing gyc76C^WT in the muscle with twi-GAL4; mef2GAL4 (F), βPS is enriched at the MTJs (F′,F″, arrow) and is also found as intracellular puncta (F,F″, arrowhead). All embryos were stained for βPS (white) and F-actin (green) with embryos in A–C,F being additionally stained for β-gal. Embryos in A-E are at stage 16 whereas the embryo in F is at stage 15. Scale bar in A and A″ represents 5 µm.