B. malayi L4 larvae (8 to 10 dpi) stained with propidium iodide -red- and phalloidin -green-. The localization of the images taken is indicated on the schematic drawings. (A,B) anterior -proximal- part, and posterior -distal- part of the female gonad respectively. (C,D) anterior -distal- part and posterior -proximal- part of the male gonad respectively. (E) Top down view of a typical anterior lateral chord. (F) Longitudinal view of a typical posterior lateral chord. DTC, distal tip cell; GC, germ cells; i., intestine; sg., somatic gonad; lc., lateral chord; lcn, lateral chord nuclei; LC, linker cell; m., muscle; ps., pseudocoelom; r., rachis; ut., uterus; v., vulva; vc, vulval cells; Wb, Wolbachia; All images are oriented with the L4 larvae anterior part to the left. Scale bar = 20 µm.