Figure 6.

Mere15 inhibits tubulin polymerization. (A) Mere15 inhibits the polymerization of cellular α-tubulin. K562 cells were untreated (a) or treated with 20 (b), 40 (c), and 80 μg/mL Mere15 (d) for 24 h. Then cells were collected, fixed, and incubated with mouse monoclonal anti-α-tubulin antibody. Cellular microtubules were observed under fluorescence microscopy. (B) Effects of Mere15 on tubulin polymerization. Polymerization of microtubules in various concentrations (0–400 μg/mL) of Mere15 was recorded continuously for 30 min by measuring the absorbance at 340 nm.