Figure 7.

Mere15 interacts with tubulin in vitro. (A) Mere15 induces intrinsic fluorescence spectra changes of tubulin. Tubulin (2 μM) was incubated with specified concentrations of Mere15 (0–300 μg/mL) for 30 min at 37 °C. The excitation wavelength was 295 nm and emission spectra were acquired by scanning from 300 to 400 nm. (B) Changes of tubulin-ANS complex fluorescence by Mere15. Tubulin (2 μM) was incubated for 30 min at 37 °C in the absence or presence of specified concentrations of Mere15 (75, 150, 300 μg/mL). Bis-ANS (15 μM) was then added, and fluorescence was measured after 15 min of incubation at 37 °C (excitation at 400 nm, emission at 430–590 nm) using a Cary Eclipse fluorescence spectrophotometer.