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Callose production in the hypocotyl epidermis of plasmolyzed zygotic embryos. (a,b) Callose deposition was not observed in the hypocotyl epidermis of untreated zygotic embryos. (c,d) Callose deposition was observed in the cytoplasm of plasmolyzed cells in the hypocotyl epidermis of zygotic embryos pretreated with 1.0 M mannitol for 12 h. Confocal laser scanning microscope bright-field images (a,c) and aniline blue fluorescent staining of callose (blue; b,d); scale bar: 50 μm.
