Figure 2.

Callose content of zygotic embryos pretreated with 1.0 M mannitol for 0–24 h after 0–8 weeks culture on PGR-free MS. The callose content of non-plasmolyzed zygotic embryos (Un-tr) was only analyzed at four stages, as they developed into plantlets after 2 weeks culture. Data are the mean ± standard error (n = 3). Means followed by the same letter within each line were not significantly different at the level of p < 0.05, as indicated by Duncan’s multiple range test.