Table 2.

Callose content of zygotic embryos pretreated with 1.0 M mannitol and different concentrations of DDG for 12 h.

DDG concentration (μM)	Callose concentration (μg/g.fw)
Untreated	7.1 a ± 1.32
control	31.3 e ± 2.13
50	19.5 c ± 1.51
100	20.3 cd ± 1.22
200	20.6 d ± 1.85
500	16.1 b ± 1.05
1000	15.5 b ± 1.62
2000	15.8 b ± 1.44

Note: Untreated zygotic embryos were not pretreated with either plasmolysis or DDG. Each value is the mean ± SE (n = 3). Means followed by the same letter within each line were not significantly different at the level of p < 0.05, as indicated by Duncan’s multiple range test.