Figure 2.

Induction of apoptosis by DADS in DU145 cells. (A) The cells were incubated with the indicated concentrations of DADS for 48 h, fixed and stained with DAPI solution. The stained nuclei were observed under a fluorescent microscope (original magnification, 400×). Scale bar is 50 μM; (B) For the analysis of DNA fragmentation, genomic DNA from cells grown under the same conditions as (A) was extracted, separated by 1.0% agarose gel electrophoresis and visualized under UV light after staining with ethidium bromide (EtBr). The DNA marker indicates the size of the fragments of the DNA ladder. The results shown are from one representative experiment of two experiments that showed similar patterns; (C) To quantify the degree of apoptosis induced by DADS, cells were evaluated by flow cytometry for sub-G1 DNA content, which represents the cells undergoing apoptotic DNA degradation. The data are the mean ± SD of the two different experiments.