Figure 3.

Effect of DADS on the levels of death receptor-related, Bcl-2 and IAP family proteins in DU145 cells. Cells were incubated with the indicated concentrations of DADS for 48 h. The cells were lysed, and the cellular proteins were then separated by SDS-polyacrylamide gels and transferred onto nitrocellulose membranes. The membranes were probed with the indicated antibodies. The proteins were visualized using an ECL detection system. Actin was used as an internal control. The numbers represent the average densitometric analyses as compared with actin in, at a minimum, two or three different experiments.