Figure 4.

Effects of DADS on the levels of MMP in DU145 cells. (A) The cells were treated with the indicated concentrations of DADS for 48 h. They were collected and incubated with JC-1 (10 μM) for 20 min at 37 °C in the dark. The cells were then washed once with PBS and analyzed by a DNA flow cytometer; (B) The results are presented as the mean ± SD of three independent experiments. The significance was determined by the Student’s t-test (*p < 0.05 vs. untreated control); (C) The cytosolic and mitochondrial proteins were extracted cells grown under the same conditions and analyzed by Western blotting using the indicated antibodies. Actin and COX4 were used as internal controls for the cytosolic and mitochondrial fractions, respectively.