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. 2012 Nov 2;13(11):14158–14171. doi: 10.3390/ijms131114158

Figure 5.

Figure 5

Activation of caspases and the degradation of PARP and β-catenin by DADS in DU145 cells. (A) Cells were treated with the indicated concentrations of DADS for 48 h. The cells were lysed, and the cellular proteins were visualized using the indicated antibodies and an ECL detection system. Actin was used as an internal control; (B) After 48 h incubation with the indicated concentrations of DADS, the cells were lysed, and aliquots (50 μg protein) were assayed for in vitro caspase-3, -8 and -9 activity using DEVD-pNA, IETD-pNA and LEHD-pNA as substrates, respectively, at 37 °C for 1 h. The released fluorescent products were measured. The data are expressed as the mean ± SD of three independent experiments. The significance was determined by the Student’s t-test (*p < 0.05 vs. untreated control).