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. 2012 Nov 2;13(11):14158–14171. doi: 10.3390/ijms131114158

Figure 6.

Figure 6

Effects of the activation of MAPKs on DADS-induced apoptosis in DU145 cells. (A) The cells were treated with DADS (500 μM) for the indicated times. They were then lysed, and equal amounts of cell lysates were resolved by SDS-polyacrylamide gels, transferred to nitrocellulose and probed with the indicated antibodies; (B) The cells were pretreated with the indicated MAPK inhibitors (SB203580, 20 μM; SP600125, 20 μM; and PD98059, 50 μM) for 1 h and then treated with DADS (500 μM) for 48 h. The percentage of sub-G1 population was evaluated by a flow cytometer. The data are the mean ± SD of two different experiments.