Table 1.
Details of 12 novel microsatellite primers developed in Abies pinsapo.
| Locus | Primer sequences (5′-3′) a | Repeat motif | Size (bp) | Ta (°C) | Na | GenBank Accession No. |
|---|---|---|---|---|---|---|
| Pin8 | F: ‡TGATTTATCCTTCGGTCTTG R: *AAAGGGCTATGTTTGAATTG |
(CTT)11 | 258 | 50 | 3 | JX473825 |
| Pin14 | F: ‡CAAATGTTGCAATGTAGGAC R: *CCGAATATCTTGCTAGTTGTC |
(AGA)8 | 249 | 50 | 1 | JX473826 |
| Pin17 | F: ‡TCCGATCCAATAGAAGATTC R: *AAAGGTTGAATCAATGATCC |
(ATTC)8 | 428 | 50 | 2 | JX473827 |
| Pin20 | F: ‡ATACAGTTATTGGCGACTCC R:*GTGAGGCGTGTGTACAATC |
(CATA)8 | 296 | 50 | 4 | JX473828 |
| Pin22 | F: *GAGTCACATGCTTTGGTGAG R: ‡TTACCACTCAAGGCCATTAC |
(CAT)7 | 108 | 50 | 1 | JX473829 |
| Pin25 | F: ‡CCCTAATTCAATGCAATTATC R: *GCATCCTGTAGAGCAACTTG |
(TATG)8 | 167 | 50 | 1 | JX473830 |
| Pin29 | F: ‡TGATTTATCCTTCGGTCTTG R: *AAAGGGCTATGTTTGAATTG |
(CTT)11 | 257 | 50 | 2 | JX473831 |
| Pin32 | F: ‡CAGCCCAAATAATTCTCTTC R: *TAGCCTTTCTTGATGGAGAG |
(CAT)7 | 254 | 50 | 2 | JX473832 |
| Pin44 | F: *GAACGATACCATTGCATCTC R: †ACATGCTTTCTATTTCCAATC |
(AC)11 | 138 | 50 | 2 | JX473833 |
| Pin48 | F: *CCATGGACTTCGGTAATATC R: ‡TCATGACAACAAGCGAGAAC |
(GT)10 | 189 | 50 | 5 | JX473834 |
| Pin49 | F: †AAGCTGGATAATGAAAGGAC R: *GCAAATTGGTCTTTAACTCC |
(AG)10 | 173 | 50 | 2 | JX473835 |
| Pin52 | F: ‡AACACCAGGCATTCACATC R: *ACTAGCTAAGCAACCACCTC |
(CA)11 | 274 | 50 | 2 | JX473836 |
Note: F = forward primer; R = reverse primer; Ta = annealing temperature; Na = number of alleles;
a
* indicates GTTT tag; † indicates CAG tag (5′-CAGTCGGGCGTCATCA-3′), ‡ indicates M13R tag (5′-GGAAACAGCTATGACCAT-3′).