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. 2012 Nov 7;13(11):14421–14433. doi: 10.3390/ijms131114421

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0).

PMC Copyright notice

Determination of the optimal reaction temperature for the KMD1 LAMP assays. LAMP products were detected on 2% agarose gel after 60-min amplification with 5 ng of the KMD1 DNA template at 60 °C (a), 63 °C (b), and 65 °C (c). The result of each reaction was verified by four replications.