Table S3.
FADS:FMN 10 mM Mg2+ | FADS:FAD 10 mM Mg2+ | FADS:ATP 10 mM Mg2+ | FADS:ATP 0 mM Mg2+ | |||||||||
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ΔG (kcal/mol) | ΔH (kcal/mol) | −TΔS (kcal/mol) | ΔG (kcal/mol) | ΔH (kcal/mol) | −TΔS (kcal/mol) | ΔG (kcal/mol) | ΔH (kcal/mol) | −TΔS (kcal/mol) | ΔG (kcal/mol) | ΔH (kcal/mol) | −TΔS (kcal/mol) | |
WT | −7.0 | −35 | 28 | −8.4 | −26 | 18 | −6.2 | −30 | 23 | −7.2 | −31 | 24 |
H28A | −6.7 | −1.4 | −5.3 | −5.9 | −3.5 | −2.4 | −4.3 | −8.3 | 4.0 | --- | --- | --- |
H28D | −7.0 | −43 | 36 | −7.8 | −42 | 35 | −5.3 | −23 | 18 | −6.0 | −6.1 | 0.1 |
H31A | −6.7 | −2.0 | −4.7 | −6.5 | −1.2 | −5.3 | −5.9 | −2.6 | −3.3 | --- | --- | --- |
H31D | −7.1 | −1.4 | −5.7 | −6.7 | −2.1 | −4.6 | −5.9 | −4.6 | −1.3 | --- | --- | --- |
N125A | −7.2 | −40 | 33 | −7.6 | −29 | 22 | −5.3 | −25 | 20 | −7.1 | −16 | 8.7 |
N125D | −6.0 | −39 | 33 | −6.7 | −24 | 17 | −5.7 | −42 | 36 | −5.5 | −30 | 25 |
R161A | −6.5 | −42 | 36 | −8.4 | −30 | 22 | −6.2 | −34 | 27 | −6.2 | −29 | 23 |
R161D | −6.6 | −5.2 | −1.4 | −8.3 | −4.9 | −3.4 | −5.7 | −7.2 | 1.5 | −5.2 | −38 | 33 |
S164A | −8.0 | −1.1 | −6.9 | −6.3 | −17 | 11 | −5.4 | −24 | 19 | −5.5 | −40 | 35 |
S164D | −7.2 | −2.6 | −4.6 | −6.2 | −64 | 58 | −5.4 | −25 | 20 | −5.4 | −15 | 9.6 |
T165A | −5.7 | −26 | 20 | −6.7 | −18 | 11 | −5.4 | −21 | 16 | −6.1 | −31 | 25 |
T165D | −7.8 | −37 | 29 | −8.8 | −22 | 13 | −5.4 | −55 | 50 | −6.7 | −6.9 | 0.2 |