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. 2012 Nov 16;13(11):15162–15176. doi: 10.3390/ijms131115162

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0).

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The expression and purification of recombinant SaPIN2b protein. (A) An SDS-PAGE gel of the GST-SaPIN2b fusion protein that was purified from the GSTrap^™ column. M: Protein Marker (Fermentas, SM0431). Lanes E1-E6: The eluate was collected in tubes 1–6 (0.5 mL/tube), and 10 μL of each eluted fraction was loaded onto the gel. The arrow indicates the size of the GST-SaPIN2b protein; (B) The SDS-PAGE gel of rSaPIN2b. M: Protein Marker (Fermentas, SM0431). Lane 1: GST-SaPIN2b that had been digested with 0.75 U/mg thrombin for three hr. Lane 2: rSaPIN2b protein was purified on an agarose-trypsin affinity column. The sizes of GST and rSaPIN2b are indicated with arrows.