Figure 2.

Cytotoxic and apoptotic effects of chalcones in HeLa cancer cells. The cells were incubated for 24 h with chalcones at the concentrations of 25 μM and 50 μM. The values represent mean ± SD of three independent experiments performed in quadruplicate (***p < 0.001 compared with control). (a) Cytotoxic activity of chalcones in HeLa cells. The percentage of cell death was measured by MTT cytotoxicity assay; (b) Apoptotic activity of chalcones in HeLa cells. Detection of apoptotic cell death by annexin V-FITC staining using flow cytometry.