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. 2012 Nov 20;13(11):15373–15386. doi: 10.3390/ijms131115373

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0).

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Double immunohistochemical staining of frozen retinal sections of P17 C57BL/6 mice. Serial sections were treated with the polyclonal antibody, CD31. A green signal indicates positive staining for vessels, and blue signals indicate nuclei. (A) Normoxia condition; (B) Hyperoxia condition; (C) Injected with PBS before being exposed to 75% oxygen; (D) Injected with TeM before being exposed to 75% oxygen. Arrows: new blood vessels protruding into the vitreous cavity (B, C). Magnification: 100×.