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. 2012 Nov 6;4(11):2806–2830. doi: 10.3390/v4112806

Figure 2.

Figure 2

Inhibition of viral mRNA translation. (a) Three different oligomers targeting the VP35 transcript (filled symbols; VP35a = 0-1-63-413, VP35b = 0-1-63-539, and VP35c = 0-1-0-1151) and an unrelated oligomer sequence (open circles; targeting L = 0-1-63-538) incubated at different concentrations in a rabbit reticulocyte assay using a VP35 luciferase fusion gene transcript. Sigmoidal curves fit to log PMO dose versus luciferase expression revealed the potency for each oligomer sequence. All samples were evaluated in triplicate and mean and standard deviation values are presented. The grey box indicates the range of values observed in untreated control samples. (b) The EC50 for 13 different oligomers targeting VP35 (0-1-63-413, 0-1-63-539, and 0-1-0-1151), VP24 (0-1-63-537, 0-1-0-1153, NG-05-0165, and NG-05-364), L (0-1-63-538 and 0-1-0-1157) and NP (0-1-0-1147, 0-1-0-1148, 0-1-0-1149, and 0-1-0-1150) are plotted with the position of the oligomer 3’-end relative to the AUG translation start site.