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A, SDS-PAGE of total and Ni-NTA-purified proteins extracted from E. coli carrying pQE30-PrCel1 (PRCEL1) and pQE30-PrCel2 (PRCEL2) vectors after induction with isopropylthio-β-galactoside; B, EGase assay of PRCEL1. Different amounts of Ni-NTA-purified protein (a, 10 ng; b,100 ng; and c, 500 ng) were dotted onto 1% agarose plates containing 0.2% CMC and stained with Congo red (1 mg/mL) (see Methods).
