Table 1.
Important surface markers for quantification of human umbilical cord blood content.
| Human CD antigens | Cell types expressed on | CD function |
|---|---|---|
| CD3 | T cells, thymocyte subset | With TCR, TCR surface expression/signal transduction |
| CD4 | Thymocyte subset, T subset, monocytes, macrophages | MHC class II coreceptor, HIV receptor, T cell differentiation/activation |
| CD8 | Thymocyte subset, T subset, NK | MHC class I coreceptor, receptor for some mutated HIV-1, T cell differentiation/activation |
| CD11c | DC, myeloid cells, NK, B, T subset | Binds CD54, fibrinogen, and iC3b |
| CD17 | Neutrophils, mono, platelets | Lactosylceramide |
| CD19 | B, FDC | Complex w/CD21 and CD81, BCR coreceptor, B cell activation/differentiation |
| CD25 | Tact, Bact, lymph progenitors | IL-2Rα, w/IL-2Rβ, and γ to form high affinity complex |
| CD34 | Haematopoietic precursors, capillary endothelial, embryonic fibroblasts | Stem cell marker, adhesion, CD62L receptor |
| CD45 | Haematopoietic cells, multiple isoforms from alternative splicing | Tyrosine phosphatase, enhanced TCR, and BCR signals |
| CD56 | NK, T subset, neurones, some large granular lymphocyte leukemias, myeloid leukemias | Adhesion |
| CD123 | Lymph subset, basophils, haematopoietic progenitors, macrophages, DC, megakaryocytes | IL-3Rα, w/CDw131 |
| CD133 | Haematopoietic stem cell subset, epithelial, endothelial | Adhesion |
| 7ADD | Nucleic acid attachment | Apoptosis marker and viability assessment |
| Lin1 | CD3; T-lymphocytes CD14; monocytes, macrophages, neutrophils and eosinophils, CD16; NK-lymphocytes, macrophages, cultured monocytes and neutrophils, CD19/CD20; B-lymphocytes, CD56; activated and resting NK-lymphocytes |
With TCR, TCR surface expression/signal transduction. Pattern recognition receptor Fc receptor B-cell coreceptor Adhesion molecule |
| HLA-DR | Macrophages, B-cells and dendritic cells | MHC class II cell surface marker |