Fig. 6.

Transfection of rat lens epithelial explants with TrkC leads to NT3-dependent fiber differentiation. (A-F) Lens epithelial explants were transfected with PLXSG retroviral vector alone (A-C) or the PLXSG/TrkC expression construct (D-F). Transfected explants were treated with FGF2 (B,E) or NT3 (C,F). Whereas FGF2 induced lens epithelial cell differentiation (as measured by elongation; B,E) in both vector- and TrkC-transfected cells, NT3-induced differentiation only occurred in TrkC-transfected explants (F). The PLXSG vector transfected cells express GFP (green), and cells were counterstained with TRITC-conjugated lectin (purple).