Fig. 6.

Irx6^lacZ/lacZ mice have a reduction in Cabp5 staining in type 3a bipolar cells. (A-L) Irx6^+/lacZ (A-F) and Irx6^lacZ/lacZ mice (G-L) were immunolabeled for Irx6:βgal (A,G), Hcn4 (B,H) and Cabp5 (C,I). Arrows indicate cells in the heterozygous retina (A-D) that are positive for Irx6:βgal, Hcn4 and Cabp5, and cells in the Irx6^lacZ/lacZ retina (G-J) that are Irx6:βgal and Hcn4 positive, but have lost Cabp5 expression. Bracket in H indicates Hcn4 staining in the inner plexiform layer of the mutant mouse. Staining for Irx6:βgal (E,K) and PKARIIβ (F,L) shows that there is no change in the colocalization of these markers with Cabp5 in the heterozygous and Irx6^lacZ/lacZ mouse. Arrows in E,F,K,L indicate cells that are positive for Cabp5 and PKARIIβ expression. Arrowheads in E,F,K,L indicate Irx6:βgal-expressing cells that are positive (E,F) or negative (K,L) for Cabp5. Scale bar: 12 μm.