Fig. 6.

Test of colocalization of each of the ten Frizzleds with Celsr1 in MDCK cells. Each of the ten mouse Frizzled-1D4 cDNAs was transiently transfected either alone or with mouse Celsr1-GFP cDNA into MDCK cells. Two days later, the subcellular distributions of the two proteins were visualized in adjacent pairs of transfected cells. For each Frizzled-1D4, two pairs of cells are shown from the transfection lacking Celsr1-GFP (first two panels in each row) followed by five pairs of cells from the transfection with Celsr1-GFP (green, top rows). Celsr1-GFP accumulates at the junction between adjacent cells, but the subcellular localization of Frizzled (red, middle rows) varies depending on the identity of the Frizzled protein. White arrowheads indicate Frizzled-1D4 co-enrichment with Celsr1-GFP at the junction between adjacent transfected cells. Bottom panel shows the merged images, with DAPI staining in blue. Scale bar: 20 μm.