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. 2012 Dec 1;139(23):4395–4404. doi: 10.1242/dev.080358

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© 2012. Published by The Company of Biologists Ltd

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Fig. 2. — Wnt/β-catenin signaling is active in cultured mouse embryonic cochlear explants. (A-D) Low- (A,C) and high- (B,D) magnification images of E13.5 cochlear explant cultures electroporated with the TOP-dGFP/pCAG-mCherry reporter construct and maintained for either 2 (A,B) or 5 (C,D) DIV. The boxed regions in A and C are magnified in B and D, respectively; medial is towards the bottom and lateral towards the top. Red (mCherry) marks transfected cells; green (TOP-dGFP) indicates cells with active TCF signaling; Sox2 (blue) is shown to indicate the prosensory/organ of Corti (OC) domain. a, apical region; b, basal region. Scale bars: 500 μm in A,C; 50 μm in B,D.