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. 2012 Dec 1;139(23):4395–4404. doi: 10.1242/dev.080358

Fig. 7.

Fig. 7.

Canonical Wnt signaling induces ectopic prosensory identity. (A-B′) Lumenal surface views showing immunocytochemistry for jagged 1 (Jag1, green) and Sox2 (red, A) or Myo6 (red, B) in E12 (A) or E13.5 (B) explants maintained for 5 DIV. Compared with controls (A,B), Jag1 expression is increased following LiCl treatment (A′,B′) and reduced following Wnt inhibition with FH535 (A″). (C,C′) Prox1 expression is also increased in E13.5 cultures maintained for 5 DIV in LiCl (C′) compared with controls (C); counterstaining is for Sox2 (red) and Myo7a (blue), with merge at left and individual channels to right. (D-D″) Immunocytochemistry for the prosensory marker p27kip1 (green) in cultures established at E13.5 and maintained for only 3 DIV in control media (D), LiCl (D′) or FH535 (D″) and counterstained for Myo6 (blue) and Sox2 (red); n>10 explants per condition per antibody. Scale bars: 50 μm in A-A″,D-D″; 100 μm in B-C′.