Figure 1.
Nuclear localization of β-catenin in CAV. (A) Frozen sections (10 μm) from naïve, isograft, and allograft murine hearts were histologically stained for elastin using Verhoeff-Van Gieson method to visualize coronary vessel occlusion. (B) Adjacent sections were immunohistochemically probed for smooth muscle actin (sma, green) – a marker of VSMCs – and β-catenin (red), and counterstained with DAPI (blue) to label nuclei. Dotted lines denoted tunica media. (C) Higher magnification of boxed area in (B) showing nuclear localization of β-catenin (arrowheads). Scale bar in A = 25 μm. Scale bar in C = 10 μm.
