Figure 3. Target Quantification with MEQ-LAMP.

(A) MEQ-LAMP reactions containing different initial copy numbers of S. Typhimurium genomic DNA (from 1.6×10⁴ to 1.6×10¹ copies) display temporally distinct local minima, revealing a clear pattern wherein samples with higher initial target copy number yield shorter t_TH. (B) The calibration curve between initial target copy number and t_TH shows a log-linear relationship that spans four orders of magnitude (from 1.6×10⁴ to 1.6×10¹ copies) with high sensitivity and reproducibility (R² = 0.91). Values and error bars represent means and standard deviations from four separate measurements.