Table 1.
Primary and secondary antibodies.
| Antibody | species | concentration | source |
|---|---|---|---|
| Rod opsin | mouse | 1 to 100 | Gift from Dr. Robert Molday, UBC |
| Chat | goat | 1 to 50 | Chemicon, Temcula, CA |
| Neurofilament | rabbit | 1 to 400 | Chemicon, Temcula, CA |
| PDE gamma | rabbit | 1 to 100 | Cytosignal, Irvine, CA |
| Calbindin | mouse | 1 to 1000 | Abcam, Cambridge, MA |
| Synaptophysin | rabbit | 1 to 400 | Dako, Carpinteria, CA |
| PKC beta | mouse | 1 to 50 | Santa Cruz Biotech |
| GFAP | rabbit | 1 to 400 | Dako, Carpinteria, CA |
| Vimentin | mouse | 1 to 500 | Dako, Carpinteria, CA |
| CtBP2 (ribeye) | mouse | 1 to 250 | BD Transduction labs, San Jose, CA |
| Biotinlyated lectins | |||
| PNA | 1 to 200 | Vector Labs, Burlingame, CA | |
| Isolectin B4 | 1 to 50 | Vector Labs | |
| Secondary Antibodies | |||
| Donkey anti-mouse, anti-rabit, anti-goat conjugated to either Cy2, Cy3 or Cy 5 | |||
| Streptavidin conjugated to either Cy2, Cy3, or Cy5 (to detect biotinylated lectins) | |||
| All from Jackson ImmunoResearch, West Grove, PA | |||
| All used at 1 to 200 concentration | |||
| Blocking serum (normal donkey serum), 1 to 20, JacksonImmunoResearch | |||