Figure 3.

IFN-γ induces a specific decrease in MyHC without altering total protein synthesis or degradation. A: Quantification of total protein synthesis measured using [³H]-tyrosine pulse assay in NRVMs treated with vehicle (control) or IFN-γ. CPM, count per minute. B: Quantification of protein degradation by measuring [³H]-tyrosine released in the media 24 hours after vehicle, IFN-γ, NH₄Cl, or MG-132 treatment. C: Myofibrillar autoradiogram of NRVM proteins pulse labeled with [³⁵S]-methionine and treated with vehicle or IFN-γ. MyHC and actin bands are as indicated. D: Quantification of MyHC and actin band intensities (C) expressed as a ratio, normalized to vehicle-treated cells. ***P < 0.001 compared with control.