Figure 1.

HemEC-P isolated from proliferating IH exhibited increased vasculogenic potential compared to HemEC-I isolated from involuting IH. A: Proliferation of HemEC-P and HemEC-I cultured in EBM-2/20% FBS over 6 days evaluated by counting cells. B: Proliferation of HemEC-P and HemEC-I cultured in EBM-2/20% FBS over 4 days evaluated by measuring cellular phosphatase activity. C: Schematic of in vivo model (note: HemECs implanted alone do not form vessels but require co-implantation with HemSCs).¹⁸D: HemSC co-injected with HemEC-I or HemEC-P. Representative photographs of Matrigel explants at day 10 after injection with corresponding histological sections stained with H&E. Arrows point to lumens filled with red blood cells (ie, perfused vessels). E: Quantification of microvessel density (MVD) as vessels/mm². Scale bar = 20 μm. Data are mean ± SEM.