FIG. 1.

Similar patterns of Fzd1 and Wnt4 mRNA regulation by hCG in granulosa cells and COCs. Quantitative RT-PCR analysis of Fzd1 (A) and Wnt4 (B) mRNA levels in granulosa cells isolated from immature eCG-treated mice without or 4, 6, 8, 10, or 12 h after the administration of an ovulatory dose of hCG. Analyses were also conducted on isolated COCs (C and D) obtained from immature eCG-treated mice at different times after hCG administration. The COCs were collected by follicle puncture (0 and 4 h), by flushing the oviducts (20 h), or by using both methods (12 h). For all analyses, n = 3–5 samples per time point, with each sample consisting of a pool of cells collected from two to five animals. All data were normalized to the housekeeping gene Rpl19 and are expressed as means ± SEM (columns and error bars). Columns that are not labeled with common letters are significantly different (P < 0.05).