FIG. 6.

Regulation of COC gene expression by FZD1, but not by WNT4. A) Quantitative RT-PCR analysis of genes in COCs from Fzd1^−/− mice and controls that are expressed in cumulus cells or oocytes whose expression is known to be regulated during COC expansion. B) COC genes whose expression was altered in Fzd1^−/− mice were analyzed by quantitative RT-PCR in Wnt4^{tm1.1Boer/tm1.1Boer};Tg (CYP19A1-cre)1Jri and relevant control mice. For all RT-PCR analyses, n = 5 samples per genotype per time point, and each sample represents a pool of cells collected from two to five animals. All data were normalized to the housekeeping gene Rpl19 and are expressed as means ± SEM (columns and error bars). *P < 0.05; **P < 0.01.