FIG. 2.

Quantitative real time-PCR of Myh11 expression at 7 (A) and 21 (B) days of age and a temporal change in Myh11 expression (C) from 7 to 21 days of age in rats treated from 1 to 6 days of age with DES, with or without coadministration of ICI or DHT. Note nearly 50%–70% reduction in Myh11 expression as a result of DES treatment and its reversal to the control level by ICI or DHT coadministration at 7 and/or 21 days. Note a temporal threefold increase in Myh11 expression in controls versus a nonsignificant onefold increase in the DES group. Temporal changes in other treatments are not shown. D) Note almost 80% reduction in MYH11 expression as a result of DES treatment. E) Western blot analysis of MYH11 protein expression at 10 days of age in control and DES-treated rats. Data are expressed as means ± SEM. Means with different lowercase letters are significantly different from each other (P < 0.05). Asterisks denote significant difference from controls at the level of *P < 0.05 or **P < 0.001. In A and B, numbers within bars indicate the number of animals in each experiment group.