FIG. 3.

Immunohistochemical localization of smooth muscle alpha actin in the body of the penis at 7, 10, 21, days of age and at adulthood in control (A–D) and DES-treated (E–H) rats. Note cavernous spaces (arrowheads) as outlined by the surrounding alpha actin-positive cavernous smooth muscle cells in the corpora cavernosa (CC). Both structures are much less developed in DES-treated animals than controls. Note arterioles or metarterioles (arrows) in DES-treated animals. Other alpha actin-positive structures are blood vessels (BV) present in the intercrural septum located dorsally. Corpus spongiosum (CS) is located ventrally. The control section incubated with blocking serum in place of primary antibody did not stain smooth muscle cells surrounding the cavernous spaces or blood vessels in the intercrural septum and is not shown. All photomicrographs were obtained at the same magnification. Bar = 100 μm.